pgex6p 1 gst vector Search Results


gst  (Cytiva Europe)
95
Cytiva Europe gst
<t>(A)</t> <t>AP2</t> structure. AD: appendage domain. (B-C) Immunoprecipitation (IP) of eGFP from naive ARPE-HPV cells (control IPs) or ARPE-HPV cells that stably express AP2-α-eGFP (AP2-α-eGFP IPs) using anti-GFP beads. (B) The domain structure of AP2-α-eGFP. (C) Volcano plot after mass spectrometry analysis of the IP samples for protein ID and abundance detection. Green: AP2 subunits; orange: known AP2 interactors. (D-F) <t>GST</t> pull-down assays. (D) Coomassie blue stained SDS-page gel of purified GST, GST-AP2-α-AD, and GST-AP2-β-AD. (E) Representative western blot result of n=3 GST pull-down assay from ARPE-HPV eGFP-CCDC32(FL) cell lysate using purified GST, GST-AP2-α-AD, or GST-AP2-β-AD. The amount of bait GST-proteins is shown in (D), and the pulled down eGFP-CCDC32 was detected by immunoblotting (IB) of GFP. (F) Relative CCDC32 enrichments quantified from immunoblotting results as shown in (E). (G-I) IP of eGFP from ARPE-HPV cells that stably express eGFP or eGFP-CCDC32(FL) using anti-GFP magnetic beads. (G) The domain structures of eGFP and eGFP-CCDC32(FL). (H) Representative immunoblotting result of n=3 IP samples. (I) Relative AP2 enrichments quantified from immunoblotting results. Error bars are SD. Two-tailed student’s t-test: ***, p ≤ 0.001.
Gst, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex6p+1+gst+vector/bio_rxiv__2024__06__26__600785-131-0-31?v=Cytiva+Europe
Average 95 stars, based on 1 article reviews
gst - by Bioz Stars, 2026-08
95/100 stars
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gst expression vector pgex6p 1  (Cytiva Europe)
96
Cytiva Europe gst expression vector pgex6p 1
<t>(A)</t> <t>AP2</t> structure. AD: appendage domain. (B-C) Immunoprecipitation (IP) of eGFP from naive ARPE-HPV cells (control IPs) or ARPE-HPV cells that stably express AP2-α-eGFP (AP2-α-eGFP IPs) using anti-GFP beads. (B) The domain structure of AP2-α-eGFP. (C) Volcano plot after mass spectrometry analysis of the IP samples for protein ID and abundance detection. Green: AP2 subunits; orange: known AP2 interactors. (D-F) <t>GST</t> pull-down assays. (D) Coomassie blue stained SDS-page gel of purified GST, GST-AP2-α-AD, and GST-AP2-β-AD. (E) Representative western blot result of n=3 GST pull-down assay from ARPE-HPV eGFP-CCDC32(FL) cell lysate using purified GST, GST-AP2-α-AD, or GST-AP2-β-AD. The amount of bait GST-proteins is shown in (D), and the pulled down eGFP-CCDC32 was detected by immunoblotting (IB) of GFP. (F) Relative CCDC32 enrichments quantified from immunoblotting results as shown in (E). (G-I) IP of eGFP from ARPE-HPV cells that stably express eGFP or eGFP-CCDC32(FL) using anti-GFP magnetic beads. (G) The domain structures of eGFP and eGFP-CCDC32(FL). (H) Representative immunoblotting result of n=3 IP samples. (I) Relative AP2 enrichments quantified from immunoblotting results. Error bars are SD. Two-tailed student’s t-test: ***, p ≤ 0.001.
Gst Expression Vector Pgex6p 1, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex6p+1+gst+vector/pmc02954829-211-11-15?v=Cytiva+Europe
Average 96 stars, based on 1 article reviews
gst expression vector pgex6p 1 - by Bioz Stars, 2026-08
96/100 stars
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glutathione s transferase gst fusion vector  (Cytiva Europe)
96
Cytiva Europe glutathione s transferase gst fusion vector
<t>(A)</t> <t>AP2</t> structure. AD: appendage domain. (B-C) Immunoprecipitation (IP) of eGFP from naive ARPE-HPV cells (control IPs) or ARPE-HPV cells that stably express AP2-α-eGFP (AP2-α-eGFP IPs) using anti-GFP beads. (B) The domain structure of AP2-α-eGFP. (C) Volcano plot after mass spectrometry analysis of the IP samples for protein ID and abundance detection. Green: AP2 subunits; orange: known AP2 interactors. (D-F) <t>GST</t> pull-down assays. (D) Coomassie blue stained SDS-page gel of purified GST, GST-AP2-α-AD, and GST-AP2-β-AD. (E) Representative western blot result of n=3 GST pull-down assay from ARPE-HPV eGFP-CCDC32(FL) cell lysate using purified GST, GST-AP2-α-AD, or GST-AP2-β-AD. The amount of bait GST-proteins is shown in (D), and the pulled down eGFP-CCDC32 was detected by immunoblotting (IB) of GFP. (F) Relative CCDC32 enrichments quantified from immunoblotting results as shown in (E). (G-I) IP of eGFP from ARPE-HPV cells that stably express eGFP or eGFP-CCDC32(FL) using anti-GFP magnetic beads. (G) The domain structures of eGFP and eGFP-CCDC32(FL). (H) Representative immunoblotting result of n=3 IP samples. (I) Relative AP2 enrichments quantified from immunoblotting results. Error bars are SD. Two-tailed student’s t-test: ***, p ≤ 0.001.
Glutathione S Transferase Gst Fusion Vector, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex6p+1+gst+vector/pmc03486443-132-16-23?v=Cytiva+Europe
Average 96 stars, based on 1 article reviews
glutathione s transferase gst fusion vector - by Bioz Stars, 2026-08
96/100 stars
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lt1  (Addgene inc)
94
Addgene inc lt1
<t>(A)</t> <t>AP2</t> structure. AD: appendage domain. (B-C) Immunoprecipitation (IP) of eGFP from naive ARPE-HPV cells (control IPs) or ARPE-HPV cells that stably express AP2-α-eGFP (AP2-α-eGFP IPs) using anti-GFP beads. (B) The domain structure of AP2-α-eGFP. (C) Volcano plot after mass spectrometry analysis of the IP samples for protein ID and abundance detection. Green: AP2 subunits; orange: known AP2 interactors. (D-F) <t>GST</t> pull-down assays. (D) Coomassie blue stained SDS-page gel of purified GST, GST-AP2-α-AD, and GST-AP2-β-AD. (E) Representative western blot result of n=3 GST pull-down assay from ARPE-HPV eGFP-CCDC32(FL) cell lysate using purified GST, GST-AP2-α-AD, or GST-AP2-β-AD. The amount of bait GST-proteins is shown in (D), and the pulled down eGFP-CCDC32 was detected by immunoblotting (IB) of GFP. (F) Relative CCDC32 enrichments quantified from immunoblotting results as shown in (E). (G-I) IP of eGFP from ARPE-HPV cells that stably express eGFP or eGFP-CCDC32(FL) using anti-GFP magnetic beads. (G) The domain structures of eGFP and eGFP-CCDC32(FL). (H) Representative immunoblotting result of n=3 IP samples. (I) Relative AP2 enrichments quantified from immunoblotting results. Error bars are SD. Two-tailed student’s t-test: ***, p ≤ 0.001.
Lt1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex6p+1+gst+vector/pmc10943585-344-9-23?v=Addgene+inc
Average 94 stars, based on 1 article reviews
lt1 - by Bioz Stars, 2026-08
94/100 stars
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pjt123 pet his6 tev tag lic cloning vector 1b addgene pjt124 pgex 6p 1 prf1 his6  (Addgene inc)
94
Addgene inc pjt123 pet his6 tev tag lic cloning vector 1b addgene pjt124 pgex 6p 1 prf1 his6
Plasmids used in this study
Pjt123 Pet His6 Tev Tag Lic Cloning Vector 1b Addgene Pjt124 Pgex 6p 1 Prf1 His6, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex6p+1+gst+vector/pmc07364042-607-48-57?v=Addgene+inc
Average 94 stars, based on 1 article reviews
pjt123 pet his6 tev tag lic cloning vector 1b addgene pjt124 pgex 6p 1 prf1 his6 - by Bioz Stars, 2026-08
94/100 stars
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pgex6p1 mcherrynanobody e coli optimized gst tagged nanobody  (Addgene inc)
93
Addgene inc pgex6p1 mcherrynanobody e coli optimized gst tagged nanobody
Plasmids used in this study
Pgex6p1 Mcherrynanobody E Coli Optimized Gst Tagged Nanobody, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex6p+1+gst+vector/pm35881049__sb2c00069_si_002-10-5-12?v=Addgene+inc
Average 93 stars, based on 1 article reviews
pgex6p1 mcherrynanobody e coli optimized gst tagged nanobody - by Bioz Stars, 2026-08
93/100 stars
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pgex-6p-1 plasmid  (GenScript corporation)
90
GenScript corporation pgex-6p-1 plasmid
Plasmids used in this study
Pgex 6p 1 Plasmid, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex6p+1+gst+vector/pm34994548__ja1c09259_si_001-44-24-40?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
pgex-6p-1 plasmid - by Bioz Stars, 2026-08
90/100 stars
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pgex 6p 1 vector  (Addgene inc)
93
Addgene inc pgex 6p 1 vector
Plasmids used in this study
Pgex 6p 1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex6p+1+gst+vector/us10723804-102-11-13?v=Addgene+inc
Average 93 stars, based on 1 article reviews
pgex 6p 1 vector - by Bioz Stars, 2026-08
93/100 stars
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bamhi  (New England Biolabs)
99
New England Biolabs bamhi
Plasmids used in this study
Bamhi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex6p+1+gst+vector/custom%40r0136%4034937945?v=New+England+Biolabs
Average 99 stars, based on 1 article reviews
bamhi - by Bioz Stars, 2026-08
99/100 stars
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pgex6p-1 vector  (Solarbio Inc)
90
Solarbio Inc pgex6p-1 vector
Plasmids used in this study
Pgex6p 1 Vector, supplied by Solarbio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex6p+1+gst+vector/pm37752705-292-9-11?v=Solarbio+Inc
Average 90 stars, based on 1 article reviews
pgex6p-1 vector - by Bioz Stars, 2026-08
90/100 stars
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ecori  (New England Biolabs)
99
New England Biolabs ecori
Plasmids used in this study
Ecori, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex6p+1+gst+vector/custom%40r0101%4034937945?v=New+England+Biolabs
Average 99 stars, based on 1 article reviews
ecori - by Bioz Stars, 2026-08
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Image Search Results


(A) AP2 structure. AD: appendage domain. (B-C) Immunoprecipitation (IP) of eGFP from naive ARPE-HPV cells (control IPs) or ARPE-HPV cells that stably express AP2-α-eGFP (AP2-α-eGFP IPs) using anti-GFP beads. (B) The domain structure of AP2-α-eGFP. (C) Volcano plot after mass spectrometry analysis of the IP samples for protein ID and abundance detection. Green: AP2 subunits; orange: known AP2 interactors. (D-F) GST pull-down assays. (D) Coomassie blue stained SDS-page gel of purified GST, GST-AP2-α-AD, and GST-AP2-β-AD. (E) Representative western blot result of n=3 GST pull-down assay from ARPE-HPV eGFP-CCDC32(FL) cell lysate using purified GST, GST-AP2-α-AD, or GST-AP2-β-AD. The amount of bait GST-proteins is shown in (D), and the pulled down eGFP-CCDC32 was detected by immunoblotting (IB) of GFP. (F) Relative CCDC32 enrichments quantified from immunoblotting results as shown in (E). (G-I) IP of eGFP from ARPE-HPV cells that stably express eGFP or eGFP-CCDC32(FL) using anti-GFP magnetic beads. (G) The domain structures of eGFP and eGFP-CCDC32(FL). (H) Representative immunoblotting result of n=3 IP samples. (I) Relative AP2 enrichments quantified from immunoblotting results. Error bars are SD. Two-tailed student’s t-test: ***, p ≤ 0.001.

Journal: bioRxiv

Article Title: CCDC32 stabilizes clathrin-coated pits and drives their invagination

doi: 10.1101/2024.06.26.600785

Figure Lengend Snippet: (A) AP2 structure. AD: appendage domain. (B-C) Immunoprecipitation (IP) of eGFP from naive ARPE-HPV cells (control IPs) or ARPE-HPV cells that stably express AP2-α-eGFP (AP2-α-eGFP IPs) using anti-GFP beads. (B) The domain structure of AP2-α-eGFP. (C) Volcano plot after mass spectrometry analysis of the IP samples for protein ID and abundance detection. Green: AP2 subunits; orange: known AP2 interactors. (D-F) GST pull-down assays. (D) Coomassie blue stained SDS-page gel of purified GST, GST-AP2-α-AD, and GST-AP2-β-AD. (E) Representative western blot result of n=3 GST pull-down assay from ARPE-HPV eGFP-CCDC32(FL) cell lysate using purified GST, GST-AP2-α-AD, or GST-AP2-β-AD. The amount of bait GST-proteins is shown in (D), and the pulled down eGFP-CCDC32 was detected by immunoblotting (IB) of GFP. (F) Relative CCDC32 enrichments quantified from immunoblotting results as shown in (E). (G-I) IP of eGFP from ARPE-HPV cells that stably express eGFP or eGFP-CCDC32(FL) using anti-GFP magnetic beads. (G) The domain structures of eGFP and eGFP-CCDC32(FL). (H) Representative immunoblotting result of n=3 IP samples. (I) Relative AP2 enrichments quantified from immunoblotting results. Error bars are SD. Two-tailed student’s t-test: ***, p ≤ 0.001.

Article Snippet: GST in a pGEX-6P-1 vector, GST-AP2-α-AD in a pGEX-2T-1 vector, and GST-AP2-β-AD in a pGEX-4T-1 vector were transfected and expressed in BL21(DE3) separately, and then affinity purified using GSTrap HP column (Cytiva).

Techniques: Immunoprecipitation, Control, Stable Transfection, Mass Spectrometry, Staining, SDS Page, Purification, Western Blot, Pull Down Assay, Magnetic Beads, Two Tailed Test

Plasmids used in this study

Journal: Molecular and Cellular Biology

Article Title: Spt5 Phosphorylation and the Rtf1 Plus3 Domain Promote Rtf1 Function through Distinct Mechanisms

doi: 10.1128/MCB.00150-20

Figure Lengend Snippet: Plasmids used in this study

Article Snippet: Plus3 domain point mutations were introduced using the Phusion site-directed mutagenesis kit (Thermo Fisher Scientific) and verified by sequencing. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Plasmid Description Reference or source pJT9 pFA6a-kanMX6-CTAP2 48 pJT48 pGEX-6P-1 Addgene pJT118 pET StrepII tag LIC cloning vector (2RT) Addgene pJT123 pET His6 TEV tag LIC cloning vector (1B) Addgene pJT124 pGEX-6P-1-Prf1-His6 (expresses GST-Prf1-His, inserted at BamHI/EcoRI) This study pJT125 pGEX-6P-1-N-Terminal domain of Prf1 (expresses GST–N-terminal domain of Prf1, inserted at BamHI/XhoI) This study pJT126 pGEX-6P-1-plus3 domain of Prf1 (expresses GST-Plus3 domain of Prf1, inserted at BamHI/XhoI) This study pJT127 pGEX-6P-1-C-Terminal domain of Prf1 (expresses GST–C-terminal domain of Prf1, inserted at BamHI/XhoI) This study pJT129 pGEX-6P-1-(+3+C) domains of Prf1 (expresses GST-+3+C fragment of Prf1, inserted at BamHI/XhoI) This study pJT130 pGEX-6P-1-(+3 domainR227A of Prf1) (expresses GST-Plus3 domain R227A point mutant, inserted at BamHI/XhoI) This study pJT131 pGEX-6P-1-(+3 domainR262E of Prf1) (express GST-Plus3 domain R262E point mutant, inserted at BamHI/XhoI) This study pJT132 pGEX-6P-1-(+3 domainR296E of Prf1) (expresses GST-Plus3 domain R296E point mutant, inserted at BamHI/XhoI) This study pJT150 pGex-6p-1-Rtf1-TAP-kanMX6 (inserted at XhoI/EagI) This study pJT152 pET His6 TEV LIC-Cdc73 (expresses His-Cdc73) This study pJT153 pET His6 TEV LIC-Leo1 (expresses His-Leo1) This study pJT154 pET His6 TEV LIC-Tpr1N (1-400aminoacids) (expresses His-Tpr1N) This study pJT161 pGex-6p-1-Rtf1-R227A-TAP-kanMX6 (inserted at XhoI/EagI) This study pJT162 pGex-6p-1-Rtf1-R262E-TAP-kanMX6 (inserted at XhoI/EagI) This study pJT163 pGex-6p-1-Rtf1-R296E-TAP-kanMX6 (inserted at XhoI/EagI) This study pJT175 pET Strep-tag vector (p118) C-terminus of Paf1 (aa 260–456) (expresses Strep-tag II–Paf1C) This study Open in a separate window Plasmids used in this study To integrate mutations into the chromosomal prf1 + locus, an ∼4.5-kb region spanning the locus and including ∼250 bp of 5′ and 3′ homology was PCR amplified from strain JT202 ( prf1-TAP :: kanMX6 ) and cloned into pGEX-6P-1 to create pJT150.

Techniques: Cloning, Plasmid Preparation, Mutagenesis, Strep-tag